Purification and antipathogenic activity of lipid transfer proteins (LTPs) from the leaves of Arabidopsis and spinach
Título de la tesis:
Purification and antipathogenic activity of lipid transfer proteins (LTPs) from the leaves of Arabidopsis and spinach
Autor/es:
Segura, Ana - Moreno, Manuel - García Olmedo, Francisco
Tipo de documento:
Artículo
Universidad:
E.T.S.I. Agrónomos (UPM)
Departamento:
Biotecnologia
Idioma:
Palabras clave:
Arabidopsis; Lipid transfer protein; Plant pathogen; Spinach; Thionin
Fecha de la defensa:
Octubre 1993-01-01
Notas:
Resumen: Two homogeneous proteins active in vitro against the bacterial pathogen Clavibacter michiganensis subsp. sepedonicus were obtained from a crude cell-wall preparation from the leaves of Columbia wild-type Arabidopsis. The N-terminal amino acid sequences of these proteins allowed their identification as lipid transfer proteins (LTP-a1, LTP-a2); the LTP1-a1 sequence was identical to that deduced from a previously described cDNA (EMBL M80566) and LTP-a2 was quite divergent (44% identical positions). These proteins were not detected in the cytoplasmic fraction by Western-blot analysis. Proteins LTP...
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